Coding

Part:BBa_K4297078

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)


dCas9

"Cas9 is an RNA-directed double-stranded DNA endonuclease from Streptococcus pyogenes.Two mutations, D10A and H840A, are introduced that could inactivate the cleavage activity of Cas9, which is often named dCas9 (dead Cas9). "


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 4121


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